Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most devastating diseases of rice (Oryza sativa L.), causing tremendous yield loss worldwide. In the present study, twenty isolates of M. oryzae were categorized into three groups based on colony colour i.e., greyish blackish, greyish and white, and in two group based on the texture of the colony as smooth and rough. All the twenty isolates produced the characteristics symptoms of spindle shaped lesion on susceptible plant. Among them, 5 isolates were found to be highly virulent, 8 were moderately virulent while, 7 were mild in nature. In phylogenetic analysis, overall two major groups were formed. The Chhattisgarh (CG-2 and CG-43) blast isolates along with Indian isolate are in one group whereas; isolates from Brazil, Kenya, Japan and China are in a separate group. The present study specified that the Indian isolates are related to each other at molecular level.