A modified technique for measurement of phosphorus concentration gradient in the flooded soil around rice root

The phosphorus (P) concentration gradients at rice rhizosphere helps to study the root induced changes in the rice soil. For measurement of vertical concentration gradients rice seeds were sown in open ended plastic cylindrical pots consisting of two chambers. The upper chamber was filled with P free sand where the rice seeds were sown, while lower chamber was filled with 500 g soils. Soils and plant roots were separated by a horizontally placed stainless steel sieve (300 meshes) in such a way that only root hair could penetrate through the sieve. In another set to obtain the radial concentration gradient of P from plant roots, stainless steel mesh made cylinder was placed vertically in pot and plant was grown in soils placed inside the central cylinder. P concentration of rice rhizosphere was measured up to a distance of 45 mm from the vicinity of roots both in the vertical and radial directions. In Vertisol the P concentration was 5.48 mg kg-1 at 9 mm and attained 6.48 mg kg-1 at a distance of 27 mm from root surface in vertical direction. Where as, in Inceptisol P con centration was 33.78 mg kg-1 in first soil section and it was increased to 38.24 mg kg-1 at 27 mm away from root surface in radial direction. The concentration gradients were found up to a distance of 27 mm from root surface when the crop was 50 days old. This rhizosphere sampling technique could be effectively used under flooded rice condition considering no inter-root competition. But it has to be validated in field condition considering inter-root competition.