The effect of natural aging on fatty acids profile in aromatic Pusa Basmati rice was studied. Lipid content and fatty acid profile of the samples were measured at an interval of 30 days. The total lipid content of the samples during the period of six months of natural aging decreased by 2.8% from that of freshly harvested samples. The major fatty acids of rice lipids were oleic acid (18:1), linoleic acid (18:2), and palmitic acid (16:0), myristic acid (14:0), stearic acid (18:0) and linolenic acid (18:3) were the other fatty acids which were also identified and quantified. The results indicated that the fatty acid profiles of rice lipids changed during aging of aromatic rice. The desirable ratio of oleic acid to linoleic acid for all the samples remained within the safe limits.