The nutritional requirements of Rajalaxmi and Ajay, two elite indica hybrid rice cultivars, were investigated using three artificial media. The results indicate that the response of genotype to media were highly significant for all the three stages of anther culture examined. Among the media, the callus induction frequencies were higher on N6 medium (34.56%) followed by MO19 (28.29%) and SK-1 (25.58%) and the callus regeneration and green plnt regeneration efficiency was also high in N6 media as compared to MO19 and SK-1. The results suggest that both genotype and culture media play a critical part in the anther culture response in indica rice.