Recombinant Inbred Lines (RILs), developed from a cross between a low arsenic accumulating rice genotype (field-mutant of Swarna) and a high As accumulating rice genotype, IR 36, were grown in farmer’s field during wet season 2008 and 2009 to understand the inheritance of As accumulation in rice. As accumulation in both grain and straw was controlled by number of genes as concluded from continuous frequency distribution pattern of As accumulation data in RILs. A separate set of genes was responsible for arsenic accumulation in straw and grain of rice as no correlation was observed between arsenic accumulation in grain and that of straw. It was concluded that genes from low accumulating parent were playing a major role in grain As accumulation but genes from both parents are contributing equally for straw As accumulation. A significant amount of transgressive segregation towards high accumulating parent, had also been observed in straw As accumulation data of RILs.