Seed Protein content in rice is one of the important nutritional parameter. In the present investigation, 15 cultivars from north-eastern region of India along with two check (high protein containing ‘ARC-10075’ and low protein containing ‘Basmati 370’) were grown during wet season 2009 at experimental farm of CRRI at Cuttack. Highest crude protein (CP) was detected in Mykachak (11.09 per cent) and Full Badam (11.06 per cent), while lowest found in Basmati 370 (6.87 per cent). CP content was found negatively associated with seed yield as well as most of the important yield attributing traits like, number of seeds panicle^-1. Therefore, more selection pressure for achieving more seed yield may negatively contribute to total seed protein. Genotypes were grouped under four clusters through D² analysis. Based on inter-cluster distances and D2 values, Khasha, RCPL-1-87-4 and Pyzum white, having highest cluster mean for number of panicles per plant, number of seeds per panicle, seed yield per plant and protein yield per plant, but lowest crude protein content, located distantly from ARC-10075 having highest cluster mean for crude protein content and 100 seed weight.