Field experiments were conducted to study the effect of Biozyme on growth, total chlorophyll content and the grain yield of rice in rice-rice cropping sequence. Experimental results revealed that the different growth attributes and total leaf chlorophyll content of rice increased with the application of biozyme in combination with chemical fertilizers. When ¼ of the recommended dose of chemical fertilizer was substituted by 20 kg Biozyme ha^-1, dry matter yield outweighed the treatment of recommended dose of chemical fertilizer alone. The highest grain yield was obtained from the treatment receiving 60 kg ha^-1 of biozyme in two equal splits at 5-7 days after transplanting and at active tillering stage along with the recommended dose of chemical fertilizer. Sole application of biozyme without chemical fertilizer did not bring about better performance.