The present study was undertaken with the prime objective of mapping the chromosomal regions associated with high iron and zinc content involving the F₂ populations derived from the cross of Samba Mahsuri with Ranbir Basmati using SSR markers derived from the genomic regions associated with iron and zinc metabolism. Out of the 35 microsatellite markers used for the parental polymorphism studies in Samba Mahsuri and Ranbir Basmati, 13 markers were polymorphic, 19 markers were monomorphic and 3 were not amplified. Most of the markers studied in the mapping experiment have shown a clear association with the trait.