Microsatellite based analysis was carried out to access the genetic relatedness among eight genotypes of rice viz., IR 58025A, Pusa 6A, KMR 3R, BR 827-35-3-1-1-1R, R 710-437-1-1, PRR 78, Swarna and MTU-1010. Of the 45 microsatellite loci analyzed, only nine were identified as polymorphic among the various genotypes. The degree of similarity ranges from one polymorphic locus to six polymorphism among the genotypes studied.