Screening of rice germplasm at Central Rice Research Institute showed that rice cv. Ramakrishna is tolerant and Annapurna is highly susceptible to the Cuttack population of the rice root-knot nematode under artificial infection. F₂ plants of the cross Annapurna/Ramakrishna showed transgressive segregation of resistance. These F₂ plants were further advanced to F₈ and F₉ by single-seed-descent method. Screening of F9 RILs under artificial infection conditions confirmed transgressive segregation and lead to identification of two lines, CR3003-184 and CR3003-1-186, highly resistant over the parent Ramakrishna both in reducing production of galls on the roots.