The present study was carried out to evaluate the response of F₁ hybrids of maintainer x maintainer crosses of rice anthers for high frequency callus induction in N6 medium and shoot and green plant regeneration in MS medium. The effect of genotype was significant (p < 0.001) for callus induction, shoot and green plant regeneration. Out of 4 inter-varietal crosses evaluated, the cross CRMS31B x CRMS24B was highly responsive for callus induction (37.83 %) as well as calli responsive for shoot regeneration (41.09 %) and green plant regeneration (15.00 %) whereas the cross CRMS32B x CRMS28B was found to be poor in callus induction (18.36 %), callus responsive for shoot regeneration (20.59 %) and also in green plant regeneration (11.58 %). The results suggest that genotypes play a critical role in the anther culture response in indica rice.