Rice gall midge is considered as one of the most important insect pests of rice.Host plant resistance assumes a central role in pest management in order to alleviate crop loss as well as increase in crop production and productivity. Eleven gall midge resistant genes have been identified out of which eight genes have been mapped using molecular markers but their use in molecular breeding programs is still limited. The resistant gene, Gm4 identified from PTB10 is an ideal candidate for deployment in gene pyramiding. In the present study, in silico analysis was performed using'Rice GE'database to scan the fine mapped region of 0.141Mb on chromosome 8 between RM547 and RM22550. This region contains 412 transcriptional active genes out of which 49 candidate genes involved in host plant resistance mechanism were identified and primers were designed.Four oligo specific genes showing resistant linked allele suggesting their possible R gene mediated role in plant-pathogen interaction were validated with the F9, mapping population of TN1/PTB10. Further Marker Assisted selection using these candidate gene markers can be an important approach to develop stable resistance against rice gall midge