The heterotrimericG-protein complex, comprising of G α , G β , and G λ subunits. It is an evolutionarily conserved-signaling molecular machine which transmits signals from transmembrane receptors to downstream target proteins. Now-a-days their functions in plant stress-signalling have been reported. Here we report the physiological function of rice G-protein λ subunit (RGG1) rice (Oryza sativa L. cv. IR64) plants under salinity stress in T₃ generation. The overexpression of CaMV35S promoter driven RGG1 in transgenic rice confers high salinity (200 mM NaCl) stress tolerance. Agronomic parameters were studied and found to be higher in the transgenic plants with respect to wild type (WT) plants.