Characterization of 45 genotypes of rice was done using fifty agro-morphological traits following Distinctiveness, Uniformity and Stability test (DUS) during Sali seasons of 2012 and 2013 at the Instructional cum Research farm of Assam Agricultural University, Jorhat. Out of 50 characters studied, 6 characters were monomorphic in nature. The rest of 44 characters, 12 characters were dimorphic and 32 were polymorphic in nature which could be used to identify the genotypes. This study will be useful for breeders, researchers and farmers to identify and choose the restoration and conservation of beneficial genes for crop improvement and also to seek protection under Protection of Plant Varieties and Farmer's Rights Act.